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Ozone Effects on Pathogens

Ozone Effects on Specific Bacteria, Viruses and Molds

Bacteria are microscopically small, single-cell creatures having a primitive structure. The bacteria body is sealed by a relatively solid-cell membrane. Ozone interferes with the metabolism of bacterium-cells, most likely through inhibiting and blocking the operation of the enzymatic control system. A sufficient amount of ozone breaks through the cell membrane, and this leads to the destruction of the bacteria.

Viruses are small, independent particles, built of crystals and macromolecules, Unlike bacteria, they multiply only within the host cell. They transform protein of the host cell into proteins of their own. Ozone destroys most viruses by diffusing through the protein coat into the nucleic acid core, resulting in damage of the viral RNA. At higher concentrations, ozone destroys the capsid, or exterior protein shell by oxidation so DNA (deoxyribonucleic acid), or RNA (ribonucleic acid) structures of the microorganism are affected.

* 1 mg/l = 1 PPM

PathogenDosage
Aspergillus Niger (Black Mount)Destroyed by 1.5 to 2 mg/I
Bacillus BacteriaDestroyed by 0.2 m/I within 30 seconds
Bacillus Anthracis (causes anthrax in sheep, cattle and pigs. Also a human pathogen)Ozone susceptible
Bacillus cereus99% destruction after 5-min at 0.12 mg/l in water
B. cereus (spores)99% destruction after 5-min at 2.3 mg/l in water
Bacillus subtilis90% reduction at 0.10-PPM for 33 minutes
Bacteriophage f299.99% destruction at 0.41 mg/l for 10-seconds in water
Botrytis cinerea3.8 mg/l for 2 minutes
Candida BacteriaOzone susceptible
Clavibacter michiganense99.99% destruction at 1.1 mg/l for 5 minutes
Cladosporium90% reduction at 0.10-PPM for 12.1 minutes
Clostridium BacteriaOzone susceptible
Clostridium Botulinum Spores. Its toxin paralyzes the central nerve system, being a poison multiplying in food and meals.0.4 to 0.5 mg/l threshold value
Coxsackie Virus A995% destruction at 0.035 mg/l for 10-seconds in water
Coxsackie Virus B599.99% destruction at 0.4 mg/l for 2.5-minutes in sludge effluent
Diphtheria PathogenDestroyed by 1.5 to 2 mg/l
Eberth Bacillus (Typhus abdomanalis). Spreads typically by aqueous infection and causes typhoid.Destroyed by 1.5 to 2 mg/l
Echo Virus 29: The virus most sensitive to ozone.After a contact time of 1 minute at 1 mg/l of ozone, 99.999% killed.
Enteric virus95% destruction at 4.1 mg/l for 29 minutes in raw wastewater
Escherichia Coli Bacteria (from feces)Destroyed by 0.2 mg/l within 30 seconds in air
E-coli (in clean water)99.99% destruction at 0.25 mg/l for 1.6 minutes
E-coli (in wastewater)99.9% destruction at 2.2 mg/l for 19 minutes
Encephalomyocarditis VirusDestroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l.
Endamoebic Cysts BacteriaOzone susceptible
Enterovirus VirusDestroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l.
Fusarium oxysporum f.sp. lycopersici1.1 mg/l for 10 minutes
Fusarium oxysporum f.sp. melonogea99.99 % destruction at 1.1 mg/l for 20 minutes
GDVII VirusDestroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l.
Hepatitis A virus99.5% reduction at 0.25 mg/l for 2-seconds in a phosphate buffer
Herpes VirusDestroyed to zero level in less than 30 seconds wit 0.1 to 0.8 mg/l.
Influenza Virus0.4 to 0.5 mg/l threshold value
Klebs-Loffler BacillusDestroyed by 1.5 to 2 mg/l
Legionella pneumophila99.99% destruction at 0.32 mg/l for 20 minutes in distilled water
Luminescent Basidiomycetes (species having no melanin pigment).Destroyed in 10 minutes at 100-PPM
Mucor piriformis3.8 mg/l for 2 minutes
Mycobacterium avium99.9% with a CT value of 0.17 in water (scientifically reviewed document)
Mycobacterium foruitum90% destruction at 0.25 mg/l for 1.6 minutes in water
Penicillium BacteriaOzone susceptible
Phytophthora parasitica3.8 mg/l for 2 minutes
Poliomyelitis Virus99.99% kill with 0.3 to 0.4 mg/l in 3-4 minutes
Poliovirus type 199.5% destruction at 0.25 mg/l for 1.6 minutes in water
Proteus BacteriaVery susceptible
Pseudomonas BacteriaVery susceptible
Rhabdovirus virusDestroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l
Salmonella BacteriaVery susceptible
Salmonella typhimurium99.99% destruction at 0.25 mg/l for 1.67 minutes in water
Schistosoma BacteriaVery susceptible
Staph epidermidis90% reduction at 0.1-ppm for 1.7 min
StaphylococciDestroyed by 1.5 to 2.0 mg/l
Stomatitis VirusDestroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l
Streptococcus BacteriaDestroyed by 0.2 mg/l within 30 seconds
Verticillium dahliae99.99 % destruction at 1.1 mg/l for 20 minutes
Vesicular VirusDestroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l
Virbrio Cholera BacteriaVery susceptible
Vicia Faba progenyOzone causes chromosome aberration and its effect is twice that observed by the action of X-rays

The effect of ozone below a certain critical concentration value is small or zero. Above this level all pathogens are eventually destroyed. This effect is called all-or-none response and the critical level the "threshold value".

Last Updated: November 10, 2014

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